Soaked some decomposing oak leaves for 36 hours in preboiled water with some yeast flakes. Stirred, put 1-2 ml into test tubes with 5 ml of malt beer (“Karamalz”, I just picked a random brand), filled up with equal amounts of 5 % vinegar and whisky (didn’t have any vodka at hand). Put a thin layer of neutral vegetable oil on top.

Should keep out the vast majority of bad microbes and provide a mostly oxygen free environment for yeast.

IIRC, I’ve also read on suigeneris brewing that oak leaf litter has a 50 - 80 % chance of catching bretanomyce, which I’d be thrilled to find.

  • plactagonic@sopuli.xyzM
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    1 month ago

    Yes it can be split somehow, but if you have to do lot of samples it can get quite long even at home.

    I stored few strains of yeasts in fridge and when I had to reinoculate them on fresh plates to keep them alive for bit longer, I had work for few hours bit time sensitively split across 2 weeks.